FEBS Letters
Volume 557, Issue 1 , Pages 133-135, 16 January 2004

In vivo formation of glutamyl-tRNAGln in Escherichia coli by heterologous glutamyl-tRNA synthetases

Edited by Lev Kisselev

  • Harold Núñez

      Affiliations

    • Programa de Biologı́a Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 838-0453, Chile
    • ,
  • Claudia Lefimil

      Affiliations

    • Programa de Biologı́a Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 838-0453, Chile
    • ,
  • Bokkee Min

      Affiliations

    • Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA
    • ,
  • Dieter Söll

      Affiliations

    • Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA
    • ,
  • Omar Orellana

      Affiliations

    • Programa de Biologı́a Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 838-0453, Chile
    • Corresponding Author InformationCorresponding author. Fax: (56)-2-735 5580

Received 21 October 2003; accepted 29 October 2003.

Abstract 

Two types of glutamyl-tRNA synthetase exist: the discriminating enzyme (D-GluRS) forms only Glu-tRNAGlu, while the non-discriminating one (ND-GluRS) also synthesizes Glu-tRNAGln, a required intermediate in protein synthesis in many organisms (but not in Escherichia coli). Testing the capacity to complement a thermosensitive E. coli gltX mutant and to suppress an E. coli trpA49 missense mutant we examined the properties of heterologous gltX genes. We demonstrate that while Acidithiobacillus ferrooxidans GluRS1 and Bacillus subtilis Q373R GluRS form Glu-tRNAGlu, A. ferrooxidans and Helicobacter pylori GluRS2 form Glu-tRNAGln in E. coli in vivo.

Keywords:  Misacylated tRNA, tRNA specificity, Glutamyl-tRNA synthetase, Glutaminyl-tRNA synthetase

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PII: S0014-5793(03)01460-1

doi:10.1016/S0014-5793(03)01460-1

FEBS Letters
Volume 557, Issue 1 , Pages 133-135, 16 January 2004

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