The complete mitochondrial genome of the yeast Kluyveromyces thermotolerans

Talla, Emmanuel; Anthouard, Véronique; Bouchier, Christiane; Frangeul, Lionel; Dujon, Bernard

doi:10.1016/j.febslet.2004.10.106

« Previous Next »FEBS Letters
Volume 579, Issue 1 , Pages 30-40, 3 January 2005

The complete mitochondrial genome of the yeast Kluyveromyces thermotolerans

Edited by Gianni Cesareni

Emmanuel Talla
- Unité de Génétique Moléculaire des Levures (URA 2171 CNRS, UFR 927 Université PM Curie), Institut Pasteur, 25 rue du Docteur Roux, F-75724 Paris Cedex 15, France
- Corresponding author. Present address: Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et Microbiologie, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Fax: +33 4 9116 8914
Véronique Anthouard
- Génoscope (UMR 8030 CNRS), 2 rue Gaston Crémieux, 91057 Evry Cedex, France
Christiane Bouchier
- Plate-forme Génomique, Pasteur Genopole Ile de France, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
Lionel Frangeul
- Plate-forme Intégration et Analyse génomiques, Pasteur Genopole Ile de France, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
Bernard Dujon
- Unité de Génétique Moléculaire des Levures (URA 2171 CNRS, UFR 927 Université PM Curie), Institut Pasteur, 25 rue du Docteur Roux, F-75724 Paris Cedex 15, France

Received 13 September 2004; received in revised form 25 October 2004; accepted 28 October 2004. published online 30 November 2004.

Abstract

We report here the complete nucleotide sequence of the 23.5-kb mitochondrial genome from the yeast Kluyveromyces thermotolerans. It encodes, all on the same DNA strand, three subunits of cytochrome oxidase (COX1, COX2 and COX3), three subunits of ATP synthetase (ATP6, ATP8 and ATP9), the apocytochrome b (COB), the ribosomal protein VAR1, 24 tRNAs, the small and large ribosomal RNAs, and the RNA subunit of RNase P. Three intronic ORFs are present within the COX1 gene group I introns. The K. thermotolerans mitochondrial genome is very similar to the Candida glabrata mitochondrial genome, as judged from clusters of gene order, gene transcription units and sequence similarities. Interestingly, the predicted secondary structure of the abnormal tRNA^Thr1 contains 10 nucleotides in its anticodon loop. This sequence is available under EMBL Accession No. AJ634268.