FEBS Letters
Volume 579, Issue 13 , Pages 2807-2810, 23 May 2005

N6-Acetyladenosine: A new modified nucleoside from Methanopyrus kandleri tRNA

Edited by Lev Kisselev

  • Anselm Sauerwald

      Affiliations

    • Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA
    • ,
  • Devarasetty Sitaramaiah

      Affiliations

    • Department of Medicinal Chemistry, University of Utah, Salt Lake City, UT 84112-5820, USA
    • ,
  • James A. McCloskey

      Affiliations

    • Department of Medicinal Chemistry, University of Utah, Salt Lake City, UT 84112-5820, USA
    • Department of Biochemistry, University of Utah, Salt Lake City, UT 84112-5820, USA
    • ,
  • Dieter Söll

      Affiliations

    • Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA
    • ,
  • Pamela F. Crain

      Affiliations

    • Department of Medicinal Chemistry, University of Utah, Salt Lake City, UT 84112-5820, USA
    • Corresponding Author InformationCorresponding author. Fax: +1 801 581 7097

Received 31 March 2005; accepted 4 April 2005. published online 21 April 2005.

Abstract 

Post-transcriptionally modified nucleosides are constituents of transfer RNA (tRNA) that are known to influence tertiary structure, stability and coding properties. Modifications in unfractionated tRNA from the phylogenetically unique archaeal methanogen Methanopyrus kandleri (optimal growth temperature 98°C) were studied using liquid chromatography-mass spectrometry to establish the extent to which they might differ from those of other methanogens. The exceptionally diverse population of nucleosides included four new nucleosides of unknown structure, and one that was characterized as N6-acetyladenosine, a new RNA constituent. The nucleoside modification pattern in M. kandleri tRNA is notably different from that of other archaeal methanogens, and is closer to that of the thermophilic crenarchaeota.

Abbreviations: ac6A, N6-acetyladenosine, Mr, relative molecular mass, LC/ESI-MS, combined liquid chromatography–electrospray ionization mass spectrometry, MS/MS, tandem mass spectrometry using two coupled mass analyzers, CID, collision-induced dissociation used in MS/MS

Keywords: Liquid chromatography-mass spectrometry, Electrospray ionization mass spectrometry, Transfer RNA, Post-transcriptional RNA modification, Adenosine, N6-Acetyl, Methanopyrus kandleri

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0014-5793(05)00474-6

doi:10.1016/j.febslet.2005.04.014

FEBS Letters
Volume 579, Issue 13 , Pages 2807-2810, 23 May 2005

Article Tools

* Image Usage & Resolution