High-resolution X-ray microtomography for three-dimensional visualization of human stem cell muscle homing

Torrente, Y.; Gavina, M.; Belicchi, M.; Fiori, F.; Komlev, V.; Bresolin, N.; Rustichelli, F.

doi:10.1016/j.febslet.2006.09.031

« Previous Next »FEBS Letters
Volume 580, Issue 24 , Pages 5759-5764, 16 October 2006

High-resolution X-ray microtomography for three-dimensional visualization of human stem cell muscle homing

Edited by Ned Mantei

Y. Torrente
- Fondazione IRCCS Ospedale Policlinico di Milano, Stem Cell Laboratory, Department of Neurological Sciences, Centro Dino Ferrari, University of Milan, Padiglione Ponti, Ospedale Policinico, Via Francesco Sforza 35, 20122 Milan, Italy
- Corresponding author. Fax: +39 02 50320430.
M. Gavina
- Fondazione IRCCS Ospedale Policlinico di Milano, Stem Cell Laboratory, Department of Neurological Sciences, Centro Dino Ferrari, University of Milan, Padiglione Ponti, Ospedale Policinico, Via Francesco Sforza 35, 20122 Milan, Italy
M. Belicchi
- Fondazione IRCCS Ospedale Policlinico di Milano, Stem Cell Laboratory, Department of Neurological Sciences, Centro Dino Ferrari, University of Milan, Padiglione Ponti, Ospedale Policinico, Via Francesco Sforza 35, 20122 Milan, Italy
F. Fiori
- Department of Sciences Applied to Complex Systems, Polytechnic University of Marche, Via Ranieri 65, 60131 Ancona, Italy
V. Komlev
- Department of Sciences Applied to Complex Systems, Polytechnic University of Marche, Via Ranieri 65, 60131 Ancona, Italy
- Institute for Physical Chemistry of Ceramics, Russian Academy of Sciences, Ozernaya 48, 119361 Moscow, Russia
N. Bresolin
- Fondazione IRCCS Ospedale Policlinico di Milano, Stem Cell Laboratory, Department of Neurological Sciences, Centro Dino Ferrari, University of Milan, Padiglione Ponti, Ospedale Policinico, Via Francesco Sforza 35, 20122 Milan, Italy
F. Rustichelli
- Department of Sciences Applied to Complex Systems, Polytechnic University of Marche, Via Ranieri 65, 60131 Ancona, Italy

Received 5 July 2006; received in revised form 29 August 2006; accepted 14 September 2006. published online 27 September 2006.

Abstract

In the perspective of clinical translation of stem cell research, it would be advantageous to develop new techniques to detect donor cells after transplantation to track their fate and thus better understand their role in regeneration of damaged and diseased tissues. In this study we use X-ray computed microtomography for three-dimensional visualization of stem cells that were labeled with magnetic nanoparticles and transplanted via intra-arterial infusion. We show that X-ray computed microtomography offers the possibility to detect with high definition and resolution human cells after transplantation, and opens new possibilities for both experimental stem cell research.

Keywords: X-ray, Micro-CT, Stem cells, Nanoparticles