| | A novel nuclear-localized protein with special adenylate kinase properties from Caenorhabditis elegansEdited by Ulrike Kutay Received 21 April 2006; received in revised form 29 May 2006; accepted 31 May 2006. published online 12 June 2006. Abstract The adrenal gland protein AD-004 like protein (ADLP) from Caenorhabditis elegans was cloned and expressed in Escherichia coli. Enzyme assays showed that ADLP has special adenylate kinase (AK) properties, with ATP and dATP as the preferred phosphate donors. In contrast to all other AK isoforms, AMP and dAMP were the preferred substrates of ADLP; CMP, TMP and shikimate acid were also good substrates. Subcellular localization studies showed a predominant nuclear localization for this protein, which is different from AK1–AK5, but similar to that of human AK6. These results suggest that ADLP is more likely a member of the AK6 family. Furthermore, RNAi experiments targeting ADLP were conducted and showed that RNAi treatment resulted in the suppression of worm growth. a National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China b Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China c College of Life Sciences, Beijing Normal University, Beijing 100875, China  Corresponding author. Fax: +86 10 62765913.
PII: S0014-5793(06)00692-2 doi:10.1016/j.febslet.2006.05.074 © 2006 Federation of European Biochemical Societies | |
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ABSTRACT
