FEBS Letters
Volume 514, Issue 1 , Pages 49-54, 6 March 2002

Malignant transformation by the eukaryotic translation initiation factor 3 subunit p48 (eIF3e)

Edited by Lev Kisselev

Department of Biological Chemistry, School of Medicine, University of California, Davis, CA 95616, USA

Received 9 November 2001; accepted 14 November 2001.

Abstract 

Several components of translation, e.g. eIF4E and PKR, are implicated in cancer. The e-subunit (p48) of mammalian initiation factor 3 is encoded by the Int6 gene, a common site for integration of the mouse mammary tumor virus genome, leading to the production of a truncated eukaryotic initiation factor-3e (eIF3e). Stable expression of a truncated eIF3e in NIH 3T3 cells causes malignant transformation by four criteria: foci formation; anchorage independent growth; accelerated growth; and lack of contact inhibition. Stable expression of full-length eIF3e does not cause transformation. The truncated eIF3e also inhibits the onset of apoptosis caused by serum starvation.

Keywords:  Protein synthesis, Translation initiation, eIF3, Int6, Malignant transformation

Abbreviations:  FCS, fetal calf serum, eIF, eukaryotic initiation factor, LOH, loss of heterozygosity

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0014-5793(02)02307-4

FEBS Letters
Volume 514, Issue 1 , Pages 49-54, 6 March 2002

Article Tools

* Image Usage & Resolution