FEBS Letters
Volume 514, Issue 1 , Pages 74-77, 6 March 2002

The role of SmpB protein in trans-translation

Edited by Lev Kisselev

  • Yoshihiro Shimizu

      Affiliations

    • Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Building FSB-401, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan
    • ,
  • Takuya Ueda

      Affiliations

    • Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Building FSB-401, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan
    • Corresponding Author InformationCorresponding author. Fax: (81)-471-36 3642

Received 12 November 2001; accepted 14 November 2001.

Abstract 

The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.

Keywords:  tmRNA, 10Sa, SsrA, SmpB, trans-Translation, Translation

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PII: S0014-5793(02)02333-5

FEBS Letters
Volume 514, Issue 1 , Pages 74-77, 6 March 2002

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