FEBS Letters
Volume 533, Complete , Pages 59-62, 2 January 2003

Changes in the LHCI aggregation state during iron repletion in the unicellular red alga Rhodella violacea

Edited by Richard Cogdell

  • Jean-Michel Doan

      Affiliations

    • Present address: Universität zu Köln, Botanisches Institut, Lehrstuhl I, Gyrhofstr. 15, D-50931 Köln, Germany.
    • ,
  • Benoı̂t Schoefs

      Affiliations

    • Present address: Plasticité et Expression des Génomes Microbiens, Université Joseph Fourier, CNRS-FRE 2383, BP 53, 38041 Grenoble cedex 09, France.
    • ,
  • Alexander V. Ruban

      Affiliations

    • Present address: Robert Hill Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK.
    • ,
  • Anne-Lise Etienne

      Affiliations

    • Corresponding Author InformationCorresponding author. Fax: (33)-1 44 32 39 35

Organismes Photosynthétiques et Environnement, UMR 8543 CNRS, Département de Biologie, ENS 46 rue d’Ulm, 75230 Paris cedex 05, France

Received 28 October 2002; received in revised form 12 November 2002; accepted 18 November 2002.

Abstract 

Red algae are well suited to study the effects of iron deficiency on light-harvesting complex for photosystem I (LHCI), since they are totally devoid of light-harvesting complex for photosystem II (LHCII). Iron starvation results in a reduction of the pigment content, an increase of the fluorescence yield and a new emission band at 705 nm in the 77 K fluorescence emission spectra. These changes reflect the accumulation of uncoupled, aggregated LHCI in iron-depleted cells. Reconnection of LHCI to de novo synthesized reaction center I (RCI) is the first event, which takes place after iron addition. The changes in the aggregation state of LHCI are likely to occur also in brown and green algae.

Keywords:  Fluorescence, Iron deficiency, Light-harvesting complex, Iron repletion, Photosystem I assembly, Rhodella violacea

Abbreviations:  β-car, β-carotene, Chl, chlorophyll, LHCI and LHCII, light-harvesting complexes for photosystems I and II, P700, photosystem I activity, PBS, phycobilisome, PSI and PSII, photosystems I and II, RCI, reaction center I, Zea, zeaxanthine

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0014-5793(02)03748-1

FEBS Letters
Volume 533, Complete , Pages 59-62, 2 January 2003

Article Tools

* Image Usage & Resolution