P66^ShcA interacts with MAPKAP kinase 2 and regulates its activity

Abstract 

Three mitogen activated protein kinase-activated protein kinase 2 (MAPKAP kinase 2, MK2) interacting proteins were identified using a yeast two-hybrid approach. ShcA, a signaling phospho-protein, human polyhomeotic 2 (HPH2), a transcriptional regulator, and highly similar to smoothelin (HSTS), which is related to the cytoskeletal associated protein smoothelin, interact specifically with MK2. The interaction of MK2 with the 66 kDa isoform of ShcA, p66^ShcA, and HPH2 was confirmed using co-immunoprecipitation. MK2 is activated with p66^ShcA co-expression and p66^ShcA is an in vitro substrate for MK2, further demonstrating their association and suggesting a biological role for p66^Shc in MK2 activation.

Keywords:  Mitogen activated protein kinase-activated protein kinase 2, p66^ShcA, Human polyhomeotic 2, Highly similar to smoothelin, Heat shock protein 27

Abbreviations:  MK2 and MAPKAP kinase 2, mitogen activated protein kinase-activated protein kinase 2, MAPK, mitogen activated protein kinase, COT/TPL2, tumor progression locus, Hsp27, heat shock protein 27, TNF-α, tumor necrosis factor-α, Shc, src homology and collagen, HPH2, human polyhomeotic 2, HSTS, highly similar to smoothelin, ROS, reactive oxygen species