FEBS Letters
Volume 576, Issue 3 , Pages 433-436, 22 October 2004

Sizing the pore of the volume-sensitive anion channel by differential polymer partitioning

Edited by Maurice Montal

  • Vadim I. Ternovsky

      Affiliations

    • Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
    • Institute of Cell Biophysics RAS, Pushchino, Moscow Region 142290, Russia
    • ,
  • Yasunobu Okada

      Affiliations

    • Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
    • ,
  • Ravshan Z. Sabirov

      Affiliations

    • Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
    • Corresponding Author InformationCorresponding author. Fax: +81-564-55-7735

Received 11 August 2004; received in revised form 15 September 2004; accepted 24 September 2004. published online 07 October 2004.

Abstract 

Partitioning of ethylene glycol and its polymeric forms into the pore of the volume-sensitive outwardly rectifying (VSOR) anion channel was studied to assess the pore size. Polyethylene glycol (PEG) PEG 200–300 (Rh=0.27−−0.53 nm) effectively suppressed the single-channel currents, whereas PEG 400–4000 (Rh=0.62−−1.91 nm) had little or no effect. Since all the molecules tested effectively decreased electric conductivity of the bulk solution, the observed differential effects between PEG 200–300 and PEG 400–4000 on the VSOR single-channel current are due to their limited partitioning into the channel lumen. The cut-off radius of the VSOR channel pore was assessed to be 0.63 nm.

Abbreviations:  VSOR, volume-sensitive outwardly rectifying, PEG, polyethylene glycol

Keywords:  Volume-sensitive anion channel, Pore size, Polymer partitioning, Volume regulation, Osmotic stress

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PII: S0014-5793(04)01180-9

doi:10.1016/j.febslet.2004.09.051

FEBS Letters
Volume 576, Issue 3 , Pages 433-436, 22 October 2004

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