FEBS Letters
Volume 579, Issue 9 , Pages 1911-1916, 28 March 2005

Similarities and differences in the transcriptional regulation of the leptin gene promoter in gastric and adipose cells

Edited by Robert Barouki

  • Hélène Goı¨ot

      Affiliations

    • INSERM 683, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France
    • Present address: Laboratoires BIOCODEX, Chemin d’Armacourt, 60200 Compiegne, France.
    • ,
  • Jean-Pierre Laigneau

      Affiliations

    • INSERM 683, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France
    • ,
  • Hélène Devaud

      Affiliations

    • INSERM 683, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France
    • ,
  • Iradj Sobhani

      Affiliations

    • CHU Henri Mondor, 51 Av. Du Gal. De Lattre de Tassigny, 94000 Créteil, France
    • ,
  • André Bado

      Affiliations

    • INSERM 683, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France
    • Corresponding Author InformationCorresponding author. Fax: +33 1 42 28 87 65

Received 14 December 2004; received in revised form 26 January 2005; accepted 4 February 2005. published online 01 March 2005.

Abstract 

The stomach was reported to synthesize and secrete leptin mainly in the gastric lumen. Gastric leptin release is markedly increased after food intake, by vagal cholinergic stimulation and by cholecystokinin and secretin. Here we show that human gastric MKN-74 cells produce leptin that increases upon challenge with cholecystokinin, insulin, glucocorticoids and all-trans retinoic acid through activation of the leptin gene promoter. In addition, we demonstrate that forskolin and BRL37344 which increased cAMP levels, fail to affect the activity of leptin gene promoter in MKN74 expressing β3-adrenoceptor cells but, induce a 2-fold decrease in this activity in adipose 3T3-L1 cells. These data described for the first time, similarities and more interestingly, differences in the regulation of the leptin gene promoter in gastric cells as compared to adipocytes.

Keywords: Stomach, Leptin, Transcriptional effect, cAMP, cholecystokinin, MKN-74 cell, Adipocyte cell

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PII: S0014-5793(05)00234-6

doi:10.1016/j.febslet.2005.02.031

FEBS Letters
Volume 579, Issue 9 , Pages 1911-1916, 28 March 2005

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