TA205, an anti-talin monoclonal antibody, inhibits integrin–talin interaction

Xing, Baodong; Thuppal, Shalini; Jedsadayanmata, Arom; Du, Xiaoping; Lam, Stephen C.-T.

doi:10.1016/j.febslet.2006.02.077

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Volume 580, Issue 8 , Pages 2027-2032, 3 April 2006

TA205, an anti-talin monoclonal antibody, inhibits integrin–talin interaction

Edited by Sandro Sonnino

Department of Pharmacology, College of Medicine, University of Illinois at Chicago, 835 South Wolcott Avenue, Chicago, IL 60612, USA

Received 18 October 2005; received in revised form 24 February 2006; accepted 28 February 2006. published online 10 March 2006.

Abstract

Talin mediates integrin signaling by binding to integrin cytoplasmic tails through its FERM domain which consists of F1, F2 and F3 subdomains. TA205, an anti-talin monoclonal antibody, disrupts actin stress fibers and focal adhesion when microinjected into fibroblasts. Here, we showed that TA205 caused an allosteric inhibition of integrin α_IIbβ₃ binding to the talin FERM domain and mapped the TA205 epitope to residues 131–150 in talin F1. Furthermore, binding of a talin rod fragment to talin head was partially inhibited by TA205. These findings suggest that talin F1 may be important in regulation of integrin binding and talin head–rod interaction.

Abbreviations: CHO, Chinese hamster ovary, FERM, band four-point-one, ezrin, radixin, moesin, ELISA, enzyme-linked immunosorbent assay, PCR, polymerase chain reaction, BSA, bovine serum albumin