FEBS Letters
Volume 580, Issue 8 , Pages 2046-2052, 3 April 2006

Suppression of inducible nitric oxide synthase by 10–23 DNAzymes in murine macrophage

Edited by Vladimir Skulachev

Comparative Genomics Unit, Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi 110 007, India

Received 28 January 2006; received in revised form 23 February 2006; accepted 2 March 2006. published online 09 March 2006.

Abstract 

iNOS mRNA of J774 murine macrophage cells was cleaved by 10–23 DNAzymes. DNAzyme target site I or translation initiation site and site II have computer predicted (MFOLD) secondary structures but site III has no secondary structure. All the three DNAzymes cleaved the short transcripts generated from cloned DNA almost with equal efficiency while cleavage efficiency is higher at site III than the other two sites on isolated iNOS mRNA. Interestingly, at intracellular level, DNAzyme targeted at translation initiation codon (site I) having secondary structure cleaved iNOS mRNA, and suppressed its activity and protein expression more efficiently than that targeted at sites II and III.

Abbreviation: iNOS, inducible nitric oxide synthase

Keywords: DNAzyme, Nitric oxide, Macrophage

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0014-5793(06)00297-3

doi:10.1016/j.febslet.2006.03.004

FEBS Letters
Volume 580, Issue 8 , Pages 2046-2052, 3 April 2006

Article Tools

* Image Usage & Resolution