CYP4A11 is repressed by retinoic acid in human liver cells
Abstract
CYP4A11, the major fatty acid ω-hydroxylase in human liver is involved in the balance of lipids, but its role and regulation are both poorly understood. We studied the effects of retinoids on the regulation of CYP4A11 in the human hepatoma cell line HepaRG. Treatment of HepaRG cells with all-trans-retinoic acid resulted in a strong decrease in CYP4A11 gene expression and apoprotein content and, furthermore, was associated with a 50% decrease in the microsomal lauric acid hydroxylation activity. Such a strong suppression of CYP4A11 expression by retinoids could have a major impact on fatty acid metabolism in the liver.
Keywords: Cytochrome P450, Retinoic acid, Human hepatoma cells, HepaRG, Fatty acid hydroxylase, Liver
Abbreviations: ATRA, all-trans-retinoic acid, Bezaf, bezafibrate, 9-cis-RA, 9-cis-retinoic acid, CYP, cytochrome P450, FAS, fatty acid synthase, GAPDH, glyceraldehyde-3-phosphate dehydrogenase, ω-LAH, lauric acid ω-hydroxylase, LTB4, leukotriene B4, PGJ2, 15-deoxyprostaglandine J2, PPAR, peroxisome proliferator-activated receptor, 2BrP, 2-bromopalmitate, RAR, retinoic acid receptor, RARE, retinoic acid response element, RXR, retinoid X receptor
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PII: S0014-5793(06)00592-8
doi:10.1016/j.febslet.2006.05.006
© 2006 Federation of European Biochemical Societies
