FEBS Letters
Volume 580, Issue 16 , Pages 3818-3822, 10 July 2006

Blue news: NTP binding properties of the blue-light sensitive YtvA protein from Bacillus subtilis

Edited by Peter Brzezinski

  • Valentina Buttani

      Affiliations

    • Department of Physics, University of Parma, Italy
    • ,
  • Aba Losi

      Affiliations

    • Department of Physics, University of Parma, Italy
    • CNR-INFM, V.le G.P. Usberti 7/A, 43100 Parma, Italy
    • Corresponding Author InformationCorresponding author. Department of Physics, University of Parma, Viale G.P. Usberti 7/A, 43100 Parma, Italy. Fax: +39 0521 905223.
    • ,
  • Eugenia Polverini

      Affiliations

    • Department of Physics, University of Parma, Italy
    • CNR-INFM, V.le G.P. Usberti 7/A, 43100 Parma, Italy
    • ,
  • Wolfgang Gärtner

      Affiliations

    • Max-Planck-Institute for Bio-Inorganic Chemistry, Muelheim an der Ruhr, Germany

Received 17 May 2006; received in revised form 1 June 2006; accepted 1 June 2006. published online 15 June 2006.

Abstract 

The blue-light sensitive protein YtvA from Bacillus subtilis is built of a photoactive, flavin-binding LOV (Light, Oxygen and Voltage) domain and a STAS domain with unknown function. Here we show that YtvA binds a fluorescent derivative of guanosine triphosphate (GTPTR) that can be displaced by both GTP or ATP. Unspecific NTP (N=G or A) binding is supported by the molecular model of YtvA-STAS. Blue-light activation of YtvA results in small and dark-reversible spectroscopic changes for GTPTR, suggesting that light-driven conformational changes are transmitted from the LOV core to the GTPTR binding site. These results support the idea that STAS domains may have a general NTP binding role and open a way to investigate the molecular functionality of YtvA-STAS.

Keywords: LOV-proteins, Blue-light photoreceptor, GTP-binding proteins, ATP-binding proteins, STAS domain, Fluorescence

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PII: S0014-5793(06)00709-5

doi:10.1016/j.febslet.2006.06.007

FEBS Letters
Volume 580, Issue 16 , Pages 3818-3822, 10 July 2006

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