Characterization of red cell membrane proteins as a function of red cell density:
Annexin VII in different forms of hereditary spherocytosis
Abstract
Fresh human blood samples were collected from healthy controls and splenectomized and unsplenectomized patients with hereditary spherocytosis due to band 3 or ankyrin and spectrin deficiency. The erythrocytes were separated into age-related fractions using self-forming Percoll density gradients. Membrane proteins were analysed by 2D electrophoresis and identified by mass spectrometry.
Annexin VII was present in reticulocytes but was then lost as the cells matured. A different pattern was found in band 3-deficient samples: annexin VII was in fact present in both mature and immature red cell membranes. Cytoskeletal anomalies may then influence the turn-over of annexin VII during erythrocyte maturation.
Keywords: Red blood cell, Membrane proteins, Proteomics, Hereditary spherocytosis, Annexin VII, Mass spectrometry
Abbreviations: 2DE, two-dimensional electrophoresis, HS, hereditary spherocytosis, LCMS/MS, liquid chromatography coupled to tandem mass spectrometry, MALDI-TOF, matrix assisted laser desorption ionisation-time of flight, MS, mass spectrometry, RBC, red blood cell
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PII: S0014-5793(06)01299-3
doi:10.1016/j.febslet.2006.10.070
© 2006 Federation of European Biochemical Societies
