| | Carnosine and its constituents inhibit glycation of low-density lipoproteins that promotes foam cell formation in vitroEdited by Sandro Sonnino Received 13 December 2006; received in revised form 23 January 2007; accepted 25 January 2007. published online 07 February 2007. Abstract Glycation of low-density lipoprotein (LDL) by reactive aldehydes, such as glycolaldehyde, can result in the cellular accumulation of cholesterol in macrophages. In this study, it is shown that carnosine, or its constituent amino acids β-alanine and l-histidine, can inhibit the modification of LDL by glycolaldehyde when present at equimolar concentrations to the modifying agent. This protective effect was accompanied by inhibition of cholesterol and cholesteryl ester accumulation in human monocyte-derived macrophages incubated with the glycated LDL. Thus, carnosine and its constituent amino acids may have therapeutic potential in preventing diabetes-induced atherosclerosis. Abbreviations: AGE, advanced glycation end products, β-Ala, β-alanine, CN, carnosine, GA, glycolaldehyde, His, histidine, HMDM, human monocyte-derived macrophages, REM, relative electrophoretic mobility a Free Radical Group, Heart Research Institute, Sydney, NSW 2050, Australia b Department of Medical and Molecular Biosciences, University of Technology, Sydney, NSW 2007, Australia c Faculty of Medicine, University of Sydney, NSW 2006, Australia  Corresponding author. Fax: +61 2 9514 8206.
PII: S0014-5793(07)00130-5 doi:10.1016/j.febslet.2007.01.082 © 2007 Federation of European Biochemical Societies | |
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ABSTRACT
