A remote but significant sequence homology between glycoside hydrolase clan GH-H and family GH31

Abstract 

Although both the α-amylase super-family, i.e. the glycoside hydrolase (GH) clan GH-H (the GH families 13, 70 and 77), and family GH31 share some characteristics, their different catalytic machinery prevents classification of GH31 in clan GH-H. A significant but remote evolutionary relatedness is, however, proposed for clan GH-H with GH31. A sequence alignment, based on the idea that residues equivalent in the primordial catalytic GH-H/GH31 (β/α)₈-barrel may not be found in the present-day GH-H and GH31 structures at strictly equivalent positions, shows remote sequence homologies covering β3, β4, β7 and β8 of the GH-H and GH31 (β/α)₈-barrels. Structure comparison of GH13 α-amylase and GH31 α-xylosidase guided alignment of GH-H and GH31 members for construction of evolutionary trees. The closest sequence relationship displayed by GH31 is to GH77 of clan GH-H.

Abbreviations: GH, glycoside hydrolase, TIM, triose phosphate isomerase

Keywords: α-Amylase, α-Xylosidase, Glycoside hydrolase family 13, Glycoside hydrolase family 31, Structural alignment, Remote homology, Evolutionary tree