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Volume 581, Issue 7, Pages 1275-1286 (3 April 2007)


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Characterisation of cyclin D1 down-regulation in coronavirus infected cells

Edited by Hans-Deiter Klenk

Sally M. Harrisona, Brian K. Dovea, Lisa Rothwellb, Pete Kaiserb, Ian Tarpeyc, Gavin Brooksd, Julian A. HiscoxaeCorresponding Author Informationemail address

Received 3 November 2006; received in revised form 30 January 2007; accepted 13 February 2007. published online 01 March 2007.

Abstract 

The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy revealed that cyclin D1 decreased in IBV-infected cells as infection progressed and inhibition studies indicated that a population of cyclin D1 could be targeted for degradation by a virus mediated pathway. In contrast to the SARS-coronavirus, IBV nucleocapsid protein did not interact with cyclin D1.

a Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK

b Institute for Animal Health (Compton Laboratory), UK

c Intervet UK Ltd., Milton Keynes, UK

d School of Pharmacy, University of Reading, Reading, UK

e Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK

Corresponding Author InformationCorresponding author. Address: Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK. Fax: +44 0 113 343 3167.

PII: S0014-5793(07)00205-0

doi:10.1016/j.febslet.2007.02.039


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