Pathogenic mitochondrial DNA-induced respiration defects in hematopoietic cells result in anemia by suppressing erythroid differentiation

Abstract 

Anemia is a symptom in patients with Pearson syndrome caused by the accumulation of mutated mitochondrial DNA (mtDNA). Such mutated mtDNAs have been detected in patients with anemia. This suggested that respiration defects due to mutated mtDNA are responsible for the anemia. However, there has been no convincing experimental evidence to confirm the pathophysiological relation between respiration defects in hematopoietic cells and expression of anemia. We address this issue by transplanting bone marrow cells carrying pathogenic mtDNA with a large-scale deletion (ΔmtDNA) into normal mice. The bone marrow-transplanted mice carried high proportion of ΔmtDNA only in hematopoietic cells, and resultant the mice suffered from macrocytic anemia. They show abnormalities of erythroid differentiation and weak erythropoietic response to a stressful condition. These observations suggest that hematopoietic cell-specific respiration defects caused by mtDNAs with pathogenic mutations are responsible for anemia by inducing abnormalities in erythropoiesis.

Abbreviations: mtDNA, mitochondrial DNA, ΔmtDNA, mitochondrial DNA with pathogenic 4696-bp deletion, mito-mice, model mice for mitochondrial diseases, B6 mice, C57BL/6J mice, WT, wild-type, BM, bone marrow, RBC, red blood cell, PHZ, phenylhydrazine

Keywords: Mitochondrial respiration defects, Anemia, Mitochondrial diseases, Bone marrow transplantation, Erythropoiesis