FEBS Letters
Volume 581, Issue 16 , Pages 3105-3110, 26 June 2007

Role of HIV-1 Vpr-induced apoptosis on the release of mitochondrial lysyl-tRNA synthetase

Edited by Lev Kisselev

  • Monika Kaminska

      Affiliations

    • Institut Pasteur, Unité de Régulation Enzymatique des Activités Cellulaires, 25 Rue du Docteur Roux, 75015 Paris, France.
    • ,
  • Mathilde Francin

      Affiliations

    • INRA, Unité Biopolymères Interactions Assemblages, Rue de la Géraudière, 44316 Nantes Cedex 03, France.
    • ,
  • Vyacheslav Shalak

      Affiliations

    • Institute of Molecular Biology and Genetics, 150 Acad. Zabolotnogo Street, 03143 Kiev, Ukraine.
    • ,
  • Marc Mirande

      Affiliations

    • Corresponding Author InformationCorresponding author. Fax: + 33 1 69 82 31 29.

Laboratoire d’Enzymologie et Biochimie Structurales, C.N.R.S., 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France

Received 6 April 2007; received in revised form 24 May 2007; accepted 28 May 2007. published online 05 June 2007.

Abstract 

Mitochondrial lysyl-tRNA synthetase (LysRS) is thought to be involved in the specific packaging of into HIV-1 viral particles. The HIV-1 auxiliary viral protein Vpr is an apoptogenic protein that affects the integrity of the mitochondrial membrane and has also been reported to interact with LysRS. In the present study, we show that HIV-1 Vpr expressed in E. coli and purified to homogeneity does not interact specifically with LysRS and does not impact its aminoacylation activity. However, we also show that the mitochondrial localization of LysRS in HeLa cells is altered after addition of Vpr in the culture medium. These results suggest that HIV-1 Vpr fulfills an essential role in the process of packaging of mitochondrial LysRS.

Keywords: HIV-1, Lysyl-tRNA synthetase, Vpr, Apoptosis, Mitochondria

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PII: S0014-5793(07)00614-X

doi:10.1016/j.febslet.2007.05.076

FEBS Letters
Volume 581, Issue 16 , Pages 3105-3110, 26 June 2007

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