FEBS Letters
Volume 581, Issue 17 , Pages 3178-3182, 10 July 2007

Crystal structure of a bacterial albumin-binding domain at 1.4Å resolution

Edited by Stuart Ferguson

  • Jacob Flyvholm Cramer

      Affiliations

    • Department of Cell and Molecular Biology, Uppsala University, Biomedical Centre, Box 596, SE-751 24 Uppsala, Sweden
    • Department of Life Sciences, Aalborg University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark
    • ,
  • Peter A. Nordberg

      Affiliations

    • Astra Tech, Aminogatan 1, SE-431 21 Mölndal, Sweden
    • ,
  • Janos Hajdu

      Affiliations

    • Department of Cell and Molecular Biology, Uppsala University, Biomedical Centre, Box 596, SE-751 24 Uppsala, Sweden
    • Stanford Linear Accelerator Center, Stanford University, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
    • ,
  • Sara Lejon

      Affiliations

    • Department of Cell and Molecular Biology, Uppsala University, Biomedical Centre, Box 596, SE-751 24 Uppsala, Sweden
    • Corresponding Author InformationCorresponding author. Fax: +46 18 511755.

Received 7 May 2007; received in revised form 1 June 2007; accepted 4 June 2007. published online 12 June 2007.

Abstract 

The albumin-binding domain, or GA module, of the peptostreptococcal albumin-binding protein expressed in pathogenic strains of Finegoldia magna is believed to be responsible for the virulence and increased growth rate of these strains. Here we present the 1.4Å crystal structure of this domain, and compare it with the crystal structure of the GA–albumin complex. An analysis of protein–protein interactions in the two crystals, and the presence of multimeric GA species in solution, indicate the GA module is “sticky”, and is capable of forming contacts with a range of protein surfaces. This might lead to interactions with different host proteins.

Abbreviations: HSA, human serum albumin, GA, protein G-like albumin-binding, NMR, nuclear magnetic resonance, rms, root mean square

Keywords: GA module, Peptostreptococcal albumin-binding protein, Human serum albumin, Bacterial mimicry, X-ray crystallography

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PII: S0014-5793(07)00650-3

doi:10.1016/j.febslet.2007.06.003

FEBS Letters
Volume 581, Issue 17 , Pages 3178-3182, 10 July 2007

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