FEBS Letters
Volume 581, Issue 18 , Pages 3533-3537, 24 July 2007

DNA architectural factor and proto-oncogene HMGA2 regulates key developmental genes in pluripotent human embryonic stem cells

Edited by Paul Bertone

  • Ou Li

      Affiliations

    • Division of Genomics and Genetics, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
    • Both authors contributed equally.
    • ,
  • Jinming Li

      Affiliations

    • Division of Structural and Computational Biology, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
    • Both authors contributed equally.
    • ,
  • Peter Dröge

      Affiliations

    • Division of Genomics and Genetics, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
    • Corresponding Author InformationCorresponding author.

Received 19 April 2007; received in revised form 25 June 2007; accepted 26 June 2007. published online 05 July 2007.

Abstract 

The high-mobility group (HMG) protein A2 has been studied mostly in the mouse where its function seems critical for embryonic cell growth and adipogenesis, leading to a pygmy phenotype with greatly reduced fat tissue in homozygous knock out mice. We showed recently that among the major HMG proteins, HMGA2 is highly expressed in two human embryonic stem (hES) cell lines. Here, we employed siRNA technology in combination with quantitative reverse transcriptase polymerase chain reaction, stem cell-specific microarray analyses, and cell proliferation assays in order to probe into HMGA2’s role(s) in pluripotent hES cells. Our results establish HMGA2 as a regulator of human genes linked to mesenchymal cell differentiation, adipogenesis, and hES cell growth.

Abbreviations: qRT-PCR, quantitative reverse transcriptase polymerase chain reaction

Keywords: High-mobility group protein, DNA architectural protein, Human embryonic stem cells, Cell proliferation, Cell differentiation

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PII: S0014-5793(07)00729-6

doi:10.1016/j.febslet.2007.06.072

FEBS Letters
Volume 581, Issue 18 , Pages 3533-3537, 24 July 2007

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