Investigating protein structural plasticity by surveying the consequence of an amino acid deletion from TEM-1 β-lactamase

Abstract 

While the deletion of an amino acid is a common mutation observed in nature, it is generally thought to be disruptive to protein structure. Using a directed evolution approach, we find that the enzyme TEM-1 β-lactamase was broadly tolerant to the deletion mutations sampled. Circa 73% of the variants analysed retained activity towards ampicillin, with deletion mutations observed in helices and strands as well as regions important for structure and function. Several deletion variants had enhanced activity towards ceftazidime compared to the wild-type TEM-1 demonstrating that removal of an amino acid can have a beneficial outcome.

Abbreviations: Δ, amino acid deleted, Amp, ampicillin, Cam, chloramphenicol, CAZ, ceftazidime, Indel, insertion-deletion, MIC, minimum inhibitory concentration

Keywords: Protein plasticity, Directed evolution, Deletion mutations, Indels, Antibiotic resistance