Mouse Prickle1 and Prickle2 are expressed in postmitotic neurons and promote neurite outgrowth

Okuda, Hiroaki; Miyata, Shingo; Mori, Yasutake; Tohyama, Masaya

doi:10.1016/j.febslet.2007.08.075

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Volume 581, Issue 24 , Pages 4754-4760, 2 October 2007

Mouse Prickle1 and Prickle2 are expressed in postmitotic neurons and promote neurite outgrowth

Edited by Jesus Avila

Hiroaki Okuda
- Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
- This two authors contributed equally to this work.
Shingo Miyata
- Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
- Department of Clinical Disorder Research, The Osaka-Hamamatsu Joint Research Center for Child Mental Development, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
- Corresponding author. Address: Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
- This two authors contributed equally to this work.
Yasutake Mori
- Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Masaya Tohyama
- Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
- Department of Clinical Disorder Research, The Osaka-Hamamatsu Joint Research Center for Child Mental Development, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

Received 26 June 2007; received in revised form 8 August 2007; accepted 29 August 2007. published online 06 September 2007.

Abstract

The Drosophila planar cell polarity (PCP) gene prickle has been previously indicated as one of the regulators of gastrulation in the early embryonic stage. However, the functional role of prickle in the brain in particular is not known. We first indicated that mouse Prickle1 and Prickle2 are continually expressed in the brain throughout the embryonic stages and are observed to be specifically expressed in the postmitotic neurons. Furthermore, Prickle1 or Prickle2 depletion effectively decreases the neurite outgrowth levels of mouse neuroblastoma Neuro2a cells. These results indicate that mouse Prickle1 and Prickle2 possibly regulate positive neurite formation during brain development.

Abbreviations: CP, cortical plate, IZ, intermediate zone, LIM, specific double zinc finger motif, PCP, planar cell polarity, PCR, polymerase chain reaction, RT, reverse transcription, siRNA, small interference RNA, SP, subplate, VZ, ventricular zone