FEBS Letters
Volume 581, Issue 26 , Pages 5122-5126, 30 October 2007

ZIC1 gene expression is controlled by DNA and histone methylation in mesenchymal proliferations

Edited by Ned Mantei

  • Rasoul Pourebrahim

      Affiliations

    • Department of Human Genetics, KU Leuven, Belgium
    • ,
  • Kim Van Dam

      Affiliations

    • Department of Human Genetics, KU Leuven, Belgium
    • ,
  • Marijke Bauters

      Affiliations

    • Department of Human Genetics, KU Leuven, Belgium
    • ,
  • Ivo De Wever

      Affiliations

    • Department of Oncology, UZ Gasthuisberg, KU Leuven, Belgium
    • ,
  • Raf Sciot

      Affiliations

    • Department of Oncological Surgery, UZ Gasthuisberg, KU Leuven, Belgium
    • ,
  • Jean-Jacques Cassiman

      Affiliations

    • Department of Human Genetics, KU Leuven, Belgium
    • ,
  • Sabine Tejpar

      Affiliations

    • Department of Human Genetics, KU Leuven, Belgium
    • Department of Gastroenterology, UZ Gasthuisberg, KU Leuven, Belgium
    • Corresponding Author InformationCorresponding author. Address: Department of Human Genetics, KU Leuven, Belgium. Fax: +32 16 345997.

Received 12 July 2007; received in revised form 26 September 2007; accepted 27 September 2007. published online 08 October 2007.

Abstract 

RNA and protein analysis revealed the consistent upregulation of the neural transcription factors ZIC1 and ZIC4 in desmoid tumors and other fibroproliferative disorders. The 5′ flanking region of the ZIC1 promoter was unmethylated in desmoid tumor fibroblasts, while a hypermethylated ZIC1 promoter was found in human and mouse cell lines not expressing the gene. In addition, expressing cells showed a H3K4me2 at the ZIC1 promoter, whereas non-expressing cells showed higher levels of H3K9me2 in the same region. To our knowledge, this is the first report describing ZIC1 expression in mesenchymal proliferations and a role for DNA methylation in the control of ZIC1 expression.

Abbreviations: ZIC, zinc finger protein of cerebellum, H3K4me2, histone H3 dimethylated at lysine 4, H3K9me2, histone H3 dimethylated at lysine 9, MEF, mouse embryonic fibroblast, TSA, trichostatin A

Keywords: Methylation, ZIC, Desmoid tumor, Fibroblasts

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PII: S0014-5793(07)01045-9

doi:10.1016/j.febslet.2007.09.061

FEBS Letters
Volume 581, Issue 26 , Pages 5122-5126, 30 October 2007

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