TRPA1 channel activation induces cholecystokinin release via extracellular calcium

Purhonen, A.K.; Louhivuori, L.M.; Kiehne, K.; Åkerman, K.E.O.; Herzig, K.H.

doi:10.1016/j.febslet.2007.12.005

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Volume 582, Issue 2 , Pages 229-232, 23 January 2008

TRPA1 channel activation induces cholecystokinin release via extracellular calcium

Edited by Maurice Montal

A.K. Purhonen
- Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland
L.M. Louhivuori
- Department of Neurobiology, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
- Institute of Biomedicine, University of Helsinki, Helsinki, Finland
K. Kiehne
- I. Department of Internal Medicine, Universitätsklinikum Schleswig Holstein (UKSH), Kiel, Germany
K.E.O. Åkerman
- Department of Neurobiology, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
- Institute of Biomedicine, University of Helsinki, Helsinki, Finland
K.H. Herzig
- Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland
- Department of Internal Medicine, Kuopio University Hospital, Kuopio, Finland
- Department of Physiology, University of Oulu, P.O. Box 5000, FIN-90014, Oulu, Finland
- Corresponding author. Address: Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland. Fax: +358 17 163030.

Received 18 October 2007; received in revised form 30 November 2007; accepted 3 December 2007. published online 13 December 2007.

Abstract

TRPA1 channels are non-selective cation channels activated by plant derived pungent products including allyl isothiocyanate (AITC) from mustard. Therefore, possible intestinal secretory functions of these channels were investigated. We detected TRPA1 mRNA in mouse and human duodenal mucosa and in intestinal mouse neuroendocrine STC-1 cells. Stimulation of STC-1 cells with AITC increased intracellular calcium ([Ca²⁺]_i) and significantly stimulated cholecystokinin secretion by 6.7-fold. AITC induced cholecystokinin release was completely blocked by TRPA1 antagonist ruthenium red and depletion of extracellular calcium and reduced by 36% by nimodipine and nifedipine. This suggests that spices in our daily food might stimulate digestive functions.

Keywords: TRP channel, TRPA1, Cholecystokinin release, Calcium