Expressed sequence tags from cynomolgus monkey (Macaca fascicularis) liver: A systematic identification of drug-metabolizing enzymes

Uno, Yasuhiro; Suzuki, Yutaka; Wakaguri, Hiroyuki; Sakamoto, Yoshiko; Sano, Hitomi; Osada, Naoki; Hashimoto, Katsuyuki; Sugano, Sumio; Inoue, Ituro

doi:10.1016/j.febslet.2007.12.031

« Previous Next »FEBS Letters
Volume 582, Issue 2 , Pages 351-358, 23 January 2008

Expressed sequence tags from cynomolgus monkey (Macaca fascicularis) liver: A systematic identification of drug-metabolizing enzymes

Edited by Takashi Gojobori

Yasuhiro Uno
- Division of Genetic Diagnosis, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
- Corresponding authors.
Yutaka Suzuki
- Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, 4-6-1 Shirokanedai, Minatoku, Tokyo 108-8639, Japan
- Corresponding authors.
Hiroyuki Wakaguri
- Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, 4-6-1 Shirokanedai, Minatoku, Tokyo 108-8639, Japan
Yoshiko Sakamoto
- Division of Genetic Diagnosis, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
Hitomi Sano
- Division of Genetic Diagnosis, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
Naoki Osada
- Department of Biomedical Resources, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan
Katsuyuki Hashimoto
- Department of Biomedical Resources, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan
Sumio Sugano
- Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, 4-6-1 Shirokanedai, Minatoku, Tokyo 108-8639, Japan
Ituro Inoue
- Division of Genetic Diagnosis, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
- Division of Molecular Life Science, School of Medicine, Tokai University, Shimokasuya 134, Isehara, Kanagawa 259-1193, Japan

Received 1 October 2007; received in revised form 14 December 2007; accepted 18 December 2007. published online 03 January 2008.

Abstract

The liver, a major organ for drug metabolism, is physiologically similar between monkeys and humans. However, the paucity of identified genes has hampered a deep understanding of drug metabolism in monkeys. To provide such a genetic resource, 28655 expressed sequence tags (ESTs) were generated from a cynomolgus monkey liver full-length enriched cDNA library, which contained 23 unique ESTs homologous to human drug-metabolizing enzymes. Our comparative genomics approach identified nine lineage-specific candidate ESTs, including three drug-metabolizing enzymes, which could be important for understanding the physiological differences between monkeys and humans.