FEBS Letters
Volume 582, Issue 3 , Pages 445-450, 6 February 2008

Mycobacterial lipomannan induces MAP kinase phosphatase-1 expression in macrophages

Edited by Laszlo Nagy

  • Elisabeth Elass

      Affiliations

    • Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille, UMR CNRS 8576, IFR 147, 59655 Villeneuve d’Ascq Cedex, France
    • Corresponding Author InformationCorresponding author. Fax: +33 3 20 43 65 55.
  • ,
  • Bernadette Coddeville

      Affiliations

    • Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille, UMR CNRS 8576, IFR 147, 59655 Villeneuve d’Ascq Cedex, France
  • ,
  • Laurent Kremer

      Affiliations

    • Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques, Université de Montpellier II et I, UMR CNRS 5235, France
    • INSERM, DIMNP Place Bataillon, 34095 Montpellier Cedex 05, France
  • ,
  • Marlène Mortuaire

      Affiliations

    • Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille, UMR CNRS 8576, IFR 147, 59655 Villeneuve d’Ascq Cedex, France
  • ,
  • Joël Mazurier

      Affiliations

    • Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille, UMR CNRS 8576, IFR 147, 59655 Villeneuve d’Ascq Cedex, France
  • ,
  • Yann Guérardel

      Affiliations

    • Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille, UMR CNRS 8576, IFR 147, 59655 Villeneuve d’Ascq Cedex, France

Received 28 November 2007; received in revised form 19 December 2007; accepted 3 January 2008. published online 15 January 2008.

Abstract 

Mycobacterial lipomannan (LM) and lipoarabinomannan (LAM) regulate macrophage activation by interacting with Toll-like receptors (TLRs). The intracellular signalling pathways elicited by these complex molecules are poorly defined. We have demonstrated that LM purified from various mycobacterial species, but not LAM from Mycobacterium kansasii or Mycobacterium bovis BCG, induced expression of the MAP kinase phosphatase 1 (MKP-1) in macrophages. Anti-TLR2 antibodies, as well as specific ERK and p38 MAPK inhibitors, decreased MKP-1 transcription in LM-stimulated cells. These findings suggest that the binding of LM to TLR2 triggers MAPK activation, followed by an up-regulation of MKP-1 expression, which in turn may act as a negative regulator of MAPK activation.

Abbreviations: LM, lipomannan, LAM, lipoarabinomannan, MKP-1, MAP kinase phosphatase 1, TLR, Toll-like receptor, MAPK, mitogen activated protein kinases, DC-SIGN, dendritic cell-specific ICAM-3 grabbing non-integrin, ManLAM, mannose-capped lipoarabinomann, PILAM, phosphoinositol-capped lipoarabinomannan

Keywords: Lipomannan, Lipoarabinomannan, Mycobacteria, MAPK, MAP kinase phosphatase-1, Toll-like receptor

 

PII: S0014-5793(08)00022-7

doi:10.1016/j.febslet.2008.01.007

FEBS Letters
Volume 582, Issue 3 , Pages 445-450, 6 February 2008