Differential epigenetic regulation of Aiolos expression in human tumoral cell lines and primary cells

Duhamel, Marianne; Navarro, Pablo; Cario-Andre, Muriel; Billot, Katy; Arrouss, Issam; Rebollo, Angelita

doi:10.1016/j.febslet.2008.01.010

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Volume 582, Issue 3 , Pages 457-467, 6 February 2008

Differential epigenetic regulation of Aiolos expression in human tumoral cell lines and primary cells

Edited by Frances Shannon

Marianne Duhamel
- Immunologie Cellulaire et Tissulaire, Hôpital Pitié-Salpêtrière, Bâtiment CERVI, U543 Inserm, 83, Bd de l’Hôpital, 75013 Paris and Université Pierre et Marie Curie, Paris VI, France
Pablo Navarro
- Institut Pasteur, Mouse Molecular Genetics Unit, 28 rue du Dr Roux, 75015 Paris, France
Muriel Cario-Andre
- Universite V Segalen, Inserm U876, 33076 Bordeaux, France
Katy Billot
- Immunologie Cellulaire et Tissulaire, Hôpital Pitié-Salpêtrière, Bâtiment CERVI, U543 Inserm, 83, Bd de l’Hôpital, 75013 Paris and Université Pierre et Marie Curie, Paris VI, France
Issam Arrouss
- Immunologie Cellulaire et Tissulaire, Hôpital Pitié-Salpêtrière, Bâtiment CERVI, U543 Inserm, 83, Bd de l’Hôpital, 75013 Paris and Université Pierre et Marie Curie, Paris VI, France
Angelita Rebollo
- Immunologie Cellulaire et Tissulaire, Hôpital Pitié-Salpêtrière, Bâtiment CERVI, U543 Inserm, 83, Bd de l’Hôpital, 75013 Paris and Université Pierre et Marie Curie, Paris VI, France
- Corresponding author. Fax: +33 142177490.

Received 28 September 2007; received in revised form 20 December 2007; accepted 10 January 2008. published online 17 January 2008.

Abstract

In order to investigate the epigenetic component of Aiolos regulation, we analyzed the methylation status of its 5′ CpG island in relation to histone modifications. Inhibition of CpG methylation restores Aiolos expression, as well as euchromatin-associated markers, in U937 and 1106 mel cell lines. DNA methylation and low levels of euchromatin-associated signatures are observed in U937 and 1106 mel cell lines, while the opposite characterizes Daudi, Jurkat, T and B cells. CpG methylation is not necessary to repress transcription in monocytes and melanocytes where silencing mechanism involves heterochromatin-associated signature. We show that DNA methylation directs Aiolos silencing and chromatin status in tumor cell lines, while in primary cells is mainly regulated by histone modifications.

Keywords: Aiolos, CpG islands, DNA methylation, Histone modifications, Transcriptional regulation