Mitochondrial function, content and ROS production in rat skeletal muscle: Effect of high-fat feeding

Hoeks, Joris; Briedé, Jacob J.; de Vogel, Johan; Schaart, Gert; Nabben, Miranda; Moonen-Kornips, Esther; Hesselink, Matthijs K.C.; Schrauwen, Patrick

doi:10.1016/j.febslet.2008.01.013

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Volume 582, Issue 4 , Pages 510-516, 20 February 2008

Mitochondrial function, content and ROS production in rat skeletal muscle: Effect of high-fat feeding

Edited by Berend Wieringa

Joris Hoeks
- Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands
- TI Food and Nutrition (TIFN), Wageningen, The Netherlands
- Corresponding author. Address: Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. Fax: +31 (0) 43 3670976.
Jacob J. Briedé
- Department of Health Risk Analysis and Toxicology, Nutrition and Toxicology Research, Institute Maastricht (NUTRIM), Maastricht University, Maastricht, The Netherlands
Johan de Vogel
- Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands
- TI Food and Nutrition (TIFN), Wageningen, The Netherlands
Gert Schaart
- Department of Movement Sciences, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Maastricht, The Netherlands
Miranda Nabben
- Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands
Esther Moonen-Kornips
- Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands
Matthijs K.C. Hesselink
- Department of Movement Sciences, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Maastricht, The Netherlands
- TI Food and Nutrition (TIFN), Wageningen, The Netherlands
Patrick Schrauwen
- Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands
- TI Food and Nutrition (TIFN), Wageningen, The Netherlands

Received 12 November 2007; received in revised form 3 January 2008; accepted 15 January 2008. published online 28 January 2008.

Abstract

A high intake of dietary fat has been suggested to diminish mitochondrial functioning in skeletal muscle, possibly attributing to muscular fat accumulation. Here we show however, that an 8-week high-fat dietary intervention did not affect intrinsic functioning of rat skeletal muscle mitochondria assessed by respirometry, neither on a carbohydrate- nor on a lipid-substrate. Interestingly, PPARGC1A protein increased by ∼2-fold upon high-fat feeding and we observed inconsistent results on different markers of mitochondrial density. Mitochondrial ROS production, assessed by electron spin resonance spectroscopy remained unaffected. Intramyocellular lipid levels increased significantly illustrating that a reduced innate mitochondrial function is not a prerequisite for intra-muscular fat accumulation.

Keywords: Skeletal muscle, Mitochondria, PGC-1, ROS, High-fat diet, Lipid