Proneural bHLH neurogenin 2 differentially regulates Nurr1-induced dopamine neuron differentiation in rat and mouse neural precursor cells in vitro

Park, Chang-Hwan; Kang, Jin Sun; Yoon, Eun-Hye; Shim, Jae-Won; Suh-Kim, Haeyoung; Lee, Sang-Hun

doi:10.1016/j.febslet.2008.01.018

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Volume 582, Issue 5 , Pages 537-542, 5 March 2008

Proneural bHLH neurogenin 2 differentially regulates Nurr1-induced dopamine neuron differentiation in rat and mouse neural precursor cells in vitro

Edited by Ned Mantei

Chang-Hwan Park
- Department of Microbiology, College of Medicine, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Institute of Mental Health, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Cell Therapy Research Center, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
Jin Sun Kang
- Department of Microbiology, College of Medicine, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Institute of Mental Health, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Cell Therapy Research Center, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
Eun-Hye Yoon
- Department of Microbiology, College of Medicine, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Institute of Mental Health, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Cell Therapy Research Center, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
Jae-Won Shim
- Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Institute of Mental Health, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Cell Therapy Research Center, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
Haeyoung Suh-Kim
- Department of Anatomy and Brain Disease Research Center, College of Medicine, Ajou University, Suwon 442-749, South Korea
Sang-Hun Lee
- Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Institute of Mental Health, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Cell Therapy Research Center, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea
- Corresponding author. Address: Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, No. 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea. Fax: +82 2 2294 6270.

Received 27 October 2007; received in revised form 13 December 2007; accepted 8 January 2008. published online 31 January 2008.

Abstract

Roles of Nurr1 and neurogenin 2 (Ngn2) have been shown in midbrain dopamine (DA) neuron development. We present here rat and mouse species-dependent differences of Nurr1 and Ngn2 actions in DA neuron differentiation. Nurr1 exogene expression caused an efficient generation of tyrosine hydroxylase (TH)-positive DA cells from rat neural precursor cells (NPCs). Nurr1-induced TH+ cell yields were low and highly variable depending on the origins of NPCs in mouse cultures. Coexpression of Ngn2 repressed Nurr1-induced generation of TH+ cells in rat cultures. In clear contrast, a robust enhancement in Nurr1-induced DA cell yields was observed in mouse NPCs by Ngn2. These findings imply that DA neurons may develop differently in the midbrains of these two species.

Keywords: Dopaminergic differentiation, Neural precursor cells, Neurogenin 2, Species-dependent differences, Nurr1