Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes

Takahashi, Michiko; Takahashi, Yutaka; Takahashi, Kenichi; Zolotaryov, Fyodor N.; Hong, Kyoung Su; Kitazawa, Riko; Iida, Keiji; Okimura, Yasuhiko; Kaji, Hidesuke; Kitazawa, Sohei; Kasuga, Masato; Chihara, Kazuo

doi:10.1016/j.febslet.2008.01.023

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Volume 582, Issue 5 , Pages 573-578, 5 March 2008

Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes

Edited by Laszlo Nagy

Michiko Takahashi
- Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-2, Kusunoki-cho, Chu-o-ku, Kobe 650-0017, Japan
Yutaka Takahashi
- Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-2, Kusunoki-cho, Chu-o-ku, Kobe 650-0017, Japan
- Corresponding author. Fax: +81 78 382 5899.
Kenichi Takahashi
- Research Affairs Center, Research Division, JCR Pharmaceuticals Co. Ltd., 2-2-10 Murotani, Nishi-ku, Kobe 651-2241, Japan
Fyodor N. Zolotaryov
- Research Affairs Center, Research Division, JCR Pharmaceuticals Co. Ltd., 2-2-10 Murotani, Nishi-ku, Kobe 651-2241, Japan
Kyoung Su Hong
- Research Affairs Center, Research Division, JCR Pharmaceuticals Co. Ltd., 2-2-10 Murotani, Nishi-ku, Kobe 651-2241, Japan
Riko Kitazawa
- Division of Molecular Pathology, Department of Biomedical Informatics, Kobe University Graduate School of Medicine, Japan
Keiji Iida
- Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-2, Kusunoki-cho, Chu-o-ku, Kobe 650-0017, Japan
Yasuhiko Okimura
- Department of Basic Allied Medicine, Kobe University School of Medicine, 7-10-2, Tomogaoka, Suma-ku, Kobe 654-0142, Japan
Hidesuke Kaji
- Division of Physiology/Metabolism, University of Hyogo, 13-71, Kita-Oji-cho, Akashi 673-8588, Japan
Sohei Kitazawa
- Division of Molecular Pathology, Department of Biomedical Informatics, Kobe University Graduate School of Medicine, Japan
Masato Kasuga
- Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-2, Kusunoki-cho, Chu-o-ku, Kobe 650-0017, Japan
Kazuo Chihara
- Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-2, Kusunoki-cho, Chu-o-ku, Kobe 650-0017, Japan

Received 15 December 2007; received in revised form 5 January 2008; accepted 14 January 2008. published online 31 January 2008.

Abstract

To explore a novel adipokine, we screened adipocyte differentiation-related gene and found that TIG2/chemerin was strongly induced during the adipocyte differentiation. Chemerin was secreted by the mature 3T3-L1 adipocytes and expressed abundantly in adipose tissue in vivo as recently described. Intriguingly, the expression of chemerin was differently regulated in the liver and adipose tissue in db/db mice. In addition, serum chemerin concentration was decreased in db/db mice. Chemerin and its receptor/ChemR23 were expressed in mature adipocytes, suggesting its function in autocrine/paracrine fashion. Finally, chemerin potentiated insulin-stimulated glucose uptake concomitant with enhanced insulin signaling in the 3T3-L1 adipocytes. These data establish that chemerin is a novel adipokine that regulates adipocyte function.

Keywords: Adipokine, Chemerin, Insulin signaling, Adipocyte differentiation, 3T3-L1 adipocyte, Insulin sensitivity