FEBS Letters
Volume 582, Issue 8 , Pages 1210-1218, 9 April 2008

Interaction between fortilin and transforming growth factor-beta stimulated clone-22 (TSC-22) prevents apoptosis via the destabilization of TSC-22

Edited by Angel Nebreda

  • Jeong Heon Lee

      Affiliations

    • Department of Obstetrics and Gynecology, Chonbuk National University Medical School, Jeonju 561-712, Republic of Korea
    • Co-first authors.
    • ,
  • Seung Bae Rho

      Affiliations

    • Research Institute, National Cancer Center, Goyang, Gyeonggi 411-769, Republic of Korea
    • Co-first authors.
    • ,
  • Sang-Yoon Park

      Affiliations

    • Research Institute, National Cancer Center, Goyang, Gyeonggi 411-769, Republic of Korea
    • ,
  • Taehoon Chun

      Affiliations

    • Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea
    • Corresponding Author InformationCorresponding author. Fax: +82 2 3290 3507.

Received 20 November 2007; received in revised form 10 January 2008; accepted 18 January 2008. published online 04 March 2008.

Abstract 

Yeast two-hybrid screening was conducted using a human ovary cDNA library to search for a novel binding protein using transforming growth factor-beta stimulated clone-22 (TSC-22). The selected protein was fortilin, which has been characterized as a nuclear anti-apoptotic protein. Overexpression of fortilin in ovarian carcinoma cells reversed TSC-22-mediated apoptosis, and the inhibition of fortilin expression via small interfering RNA (siRNA) resulted in an increase in the apoptosis of ovarian carcinoma cells. Moreover, fortilin overexpression promoted the degradation of TSC-22. Thus, an interaction between fortilin and TSC-22 prevents apoptosis via the destabilization of TSC-22 in ovarian carcinoma cells.

Structured summary


MINT-6173230, MINT-6173253:TSC22 (uniprotkb:Q15714) physically interacts (MI:0218) with fortilin (uniprotkb:P13693) by co-immunoprecipitation (MI:0019)

MINT-6173217:TSC22 (uniprotkb:Q15714) binds (MI:0407) fortilin (uniprotkb:P13693) by pull-down (MI:0096)

MINT-6173240, MINT-6173270:TSC22 (uniprotkb:Q15714) physically interacts (MI:0218) with fortilin (uniprotkb:P13693) by two-hybrid (MI:0018)

Abbreviations: DAPI, 4,6′-diamidino-2-phenylindole, GST, glutathione S-transferase, ONPG, o-nitrophenyl β-d-galactopyranoside, sifortilin, siRNA, small interfering RNA, THG-1, TSC-22 homologous gene-1, TSC-22, transforming growth factor-beta stimulated clone-22

Keywords: Apoptosis, Fortilin, Ovarian carcinoma cell, TSC-22, Yeast two hybrid

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PII: S0014-5793(08)00171-3

doi:10.1016/j.febslet.2008.01.066

Refers to corrigendum:

  • Corrigendum to “Interaction between fortilin and transforming growth factor-beta stimulated clone-22 (TSC-22) prevents apoptosis via the destabilization of TSC-22” [FEBS Lett. 582 (2008) 1210–1218] , 11 February 2009

    Jeong Heon Lee, Seung Bae Rho, Sang-Yoon Park, Taehoon Chun
    FEBS Letters 4 March 2009 (Vol. 583, Issue 5, Pages 950-951)

FEBS Letters
Volume 582, Issue 8 , Pages 1210-1218, 9 April 2008

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