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Volume 582, Issue 9, Pages 1307-1312 (16 April 2008)


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Extracellular catalase activity protects cysteine cathepsins from inactivation by hydrogen peroxide

Edited by David Lambeth

Virginie Hervé-Grépinet12, Florian Veillard1, Emmanuel Godat3, Nathalie Heuzé-Vourc’h, Fabien Lecaille, Gilles LalmanachCorresponding Author Informationemail address

Received 12 December 2007; received in revised form 8 February 2008; accepted 5 March 2008. published online 14 March 2008.

Abstract 

The resistance of secreted cysteine cathepsins to peroxide inactivation was evaluated using as model THP-1 cells. Differentiated cells released mostly cathepsin B, but also cathepsins H, K, and L, with a maximum of endopeptidase activity at day 6. Addition of non-cytotoxic concentrations of H2O2 did not affect mRNA expression levels and activity of cathepsins, while the catalase activity remained also unchanged, consistently with RT-PCR analysis. Conversely inhibition of extracellular catalase led to a striking inactivation of secreted cysteine cathepsins by H2O2. This report suggests that catalase may participate in the protection of extracellular cysteine proteases against peroxidation.

INSERM U 618, Protéases et Vectorisation Pulmonaires, Equipe Protéases et Pathologies Pulmonaires, Tours F-37000, France

Université François Rabelais, Tours F-37000, France

IFR 135, Tours F-37000, France

Corresponding Author InformationCorresponding author. Present address: INSERM U 618, Protéases et Vectorisation Pulmonaires, Université François Rabelais, Faculté de Médecine, 10 Bd Tonnellé, F-37032 Tours Cedex, France. Fax: +33 2 47 36 60 46.

1 These two authors have equally contributed to the work.

2 Current address: INRA, UR 83 Recherches Avicoles, Nouzillly, France.

3 Current address: Laboratoire de PhysioGénomique, SBGM/DBJC, CEA/Saclay, Gif-sur-Yvette, France.

PII: S0014-5793(08)00210-X

doi:10.1016/j.febslet.2008.03.007


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