FEBS Letters
Volume 582, Issue 9 , Pages 1325-1329, 16 April 2008

Sendai virus F glycoprotein induces IL-6 production in dendritic cells in a fusion-independent manner

Edited by Masayuki Miyasaka

  • Hayato Suzuki

      Affiliations

    • Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan
    • ,
  • Masayuki Kurooka

      Affiliations

    • Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan
    • ,
  • Yoko Hiroaki

      Affiliations

    • Department of Biophysics, Faculty of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
    • ,
  • Yoshinori Fujiyoshi

      Affiliations

    • Department of Biophysics, Faculty of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
    • ,
  • Yasufumi Kaneda

      Affiliations

    • Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan
    • Corresponding Author InformationCorresponding author. Fax: +81 6 6879 3909.

Received 24 January 2008; received in revised form 29 February 2008; accepted 11 March 2008. published online 19 March 2008.

Abstract 

We previously reported that inactivated Sendai virus particle (hemagglutinating virus of Japan envelope; HVJ-E) has anti-tumor effects by eliciting IL-6 production in dendritic cells (DCs). In the present study, we investigated which components of HVJ-E elicit IL-6 production. HVJ-E containing F0 protein inactive for virus envelope–cell membrane fusion enhanced IL-6 production. Reconstituted liposomes containing F protein stimulated IL-6 production. The antibody against F protein inhibited IL-6 secretion by HVJ-E. When carbohydrate chains of the F glycoprotein were removed, HVJ-E lost the ability to stimulate IL-6 secretion. These results suggest that F glycoprotein is required for IL-6 production in DCs.

Abbreviations: HVJ, hemagglutinating virus of Japan, DCs, dendritic cells, TLR, toll-like receptor, IFN, type I interferon, UV, ultra-violet rays, MOI, multiplicity of infection, HAU, hemagglutinating unit, PNGF, peptide-N-glycosidase F, EndoH, endoglycosidase H, Rev, reconstituted virosome, Rel, reconstituted liposomes

Keywords: HVJ, F protein, Carbohydrate chain, Dendritic cell, IL-6

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PII: S0014-5793(08)00240-8

doi:10.1016/j.febslet.2008.03.011

FEBS Letters
Volume 582, Issue 9 , Pages 1325-1329, 16 April 2008

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