Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

Takano, Hirotsugu; Nakazawa, Shunsuke; Okuno, Yasushi; Shirata, Naritoshi; Tsuchiya, Sohken; Kainoh, Takayuki; Takamatsu, Seigoh; Furuta, Kazuyuki; Taketomi, Yoshitaka; Naito, Yuko; Takematsu, Hiromu; Kozutsumi, Yasunori; Tsujimoto, Gozoh; Murakami, Makoto; Kudo, Ichiro; Ichikawa, Atsushi; Nakayama, Kazuhisa; Sugimoto, Yukihiko; Tanaka, Satoshi

doi:10.1016/j.febslet.2008.03.033

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Volume 582, Issue 10 , Pages 1444-1450, 30 April 2008

Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

Edited by Beat Imhof

Hirotsugu Takano
- Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Shunsuke Nakazawa
- Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Yasushi Okuno
- Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Naritoshi Shirata
- Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Sohken Tsuchiya
- Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Takayuki Kainoh
- Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Seigoh Takamatsu
- Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Kazuyuki Furuta
- Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Yoshitaka Taketomi
- Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
Yuko Naito
- Laboratory of Membrane Biochemistry and Biophysics, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Hiromu Takematsu
- Laboratory of Membrane Biochemistry and Biophysics, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Yasunori Kozutsumi
- Laboratory of Membrane Biochemistry and Biophysics, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Gozoh Tsujimoto
- Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Makoto Murakami
- Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
- Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan
Ichiro Kudo
- Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
Atsushi Ichikawa
- Institute for Biosciences, Mukogawa Women’s University, Koshien, Nishinomiya, Hyogo 663-8179, Japan
Kazuhisa Nakayama
- Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Yukihiko Sugimoto
- Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Satoshi Tanaka
- Institute for Biosciences, Mukogawa Women’s University, Koshien, Nishinomiya, Hyogo 663-8179, Japan
- Department of Immunobiology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women’s University, 11-68 Kyuban-cho, Koshien, Nishinomiya, Hyogo 663-8179, Japan
- Corresponding author. Address: Department of Immunobiology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women’s University, 11-68 Kyuban-cho, Koshien, Nishinomiya, Hyogo 663-8179, Japan. Fax: +81 798 41 2792.

Received 16 January 2008; received in revised form 11 March 2008; accepted 20 March 2008. published online 31 March 2008.

Abstract

To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.

Abbreviations: BMMC, bone marrow-derived mast cell, CTMC, connective tissue mast cell, MMC, mucosal mast cell

Keywords: Mast cell, Gene expression, Differentiation, G_i