FEBS Letters
Volume 582, Issue 10 , Pages 1413-1418, 30 April 2008

Parkin-co-regulated gene (PACRG) product interacts with tubulin and microtubules

Edited by Judit Ovádi

  • Takashi Ikeda

      Affiliations

    • Corresponding Author InformationFax: +81 42 739 8453.
    • Present address: Honeybee Science Research Center, Tamagawa University Research Institute, Machida, Tokyo 194-8610, Japan.

Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

Received 7 December 2007; received in revised form 18 February 2008; accepted 25 February 2008. published online 01 April 2008.

Abstract 

Parkin-co-regulated gene (PACRG) is a gene that shares a bidirectional promoter with Parkinson’s disease-related Parkin/Park2 gene. Recently, the PACRG gene product was implicated in the function of flagella. However, its exact function remains unknown. Here, I assessed the interaction between PACRG and tubulin. Co-sedimentation experiments revealed that PACRG directly binds to microtubules and α/β-tubulin heterodimers with high affinity. Microscopic studies showed that PACRG bundles microtubules and forms branched aggregates with unpolymerized tubulin dimers. The amino acid sequence of the microtubule-binding region of PACRG is highly conserved among various organisms, suggesting that tubulin binding is a basic property of PACRG.

Structured summary


MINT-6439731, MINT-6439753, MINT-6439768:

PACRG (genbank_protein_gi:169219363), tubulin alpha (uniprotkb:P02550), tubulin beta (uniprotkb:Q2XVP4) bind (MI:0407) by cosedimentation (MI:0027)

MINT-6439796:

PACRG (genbank_protein_gi:169219363), tubulin alpha (uniprotkb:P02550), tubulin beta (uniprotkb:Q2XVP4) bind (MI:0407) by pull-down (MI:0096)

Keywords: Axoneme, Outer doublet microtubule, Parkinson’s disease, Park2, Microtubule bundle, Chlamydomonas

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PII: S0014-5793(08)00272-X

doi:10.1016/j.febslet.2008.02.081

FEBS Letters
Volume 582, Issue 10 , Pages 1413-1418, 30 April 2008

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