Detection of cross-links between FtsH, YidC, HflK/C suggests a linked role for these proteins in quality control upon insertion of bacterial inner membrane proteins

van Bloois, Edwin; Dekker, Henk L.; Fröderberg, Linda; Houben, Edith N.G.; Urbanus, Malene L.; de Koster, Chris G.; de Gier, Jan-Willem; Luirink, Joen

doi:10.1016/j.febslet.2008.02.082

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Volume 582, Issue 10 , Pages 1419-1424, 30 April 2008

Detection of cross-links between FtsH, YidC, HflK/C suggests a linked role for these proteins in quality control upon insertion of bacterial inner membrane proteins

Edited by Stuart Ferguson

Edwin van Bloois
- Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
Henk L. Dekker
- Laboratory for Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, WV Amsterdam, The Netherlands
Linda Fröderberg
- Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, SE-106 91 Stockholm, Sweden
Edith N.G. Houben
- Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
Malene L. Urbanus
- Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
Chris G. de Koster
- Laboratory for Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, WV Amsterdam, The Netherlands
Jan-Willem de Gier
- Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, SE-106 91 Stockholm, Sweden
Joen Luirink
- Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
- Corresponding author. Fax: +31 20 5986979.

Received 22 January 2008; received in revised form 14 February 2008; accepted 15 February 2008. published online 01 April 2008.

Abstract

Little is known about the quality control of proteins upon integration in the inner membrane of Escherichia coli. Here, we demonstrate that YidC and FtsH are adjacent to a nascent, truncated membrane protein using in vitro photo cross-linking. YidC plays a critical but poorly understood role in the biogenesis of E. coli inner membrane proteins (IMPs). FtsH functions as a membrane chaperone and protease. Furthermore, we show that FtsH and its modulator proteins HflK and HflC copurify with tagged YidC and, vice versa, that YidC copurifies with tagged FtsH. These results suggest that FtsH and YidC have a linked role in the quality control of IMPs.

Structured summary

MINT-6478034:

hflB (uniprotkb:P0AAI3) physically interacts (MI:0218) with yidC (uniprotkb:P25714), hflK (uniprotkb:P0ABC7), hflC (uniprotkb:P0ABC3) by cross-linking studies (MI:0030)

MINT-6478363:

yidC (uniprotkb:P25714) physically interacts (MI:0218) with lldD (uniprotkb:P33232), rplD (uniprotkb:P60723), yrbD (uniprotkb:P64604), rpsA (uniprotkb:P0AG67), aphA1 (uniprotkb:P00551), dacC (uniprotkb:P08506), rpsC (uniprotkb:P0A7V3), rpsD (uniprotkb:P0A7V8), rpsE (uniprotkb:P0A7W1), rpoA (uniprotkb:P0A7Z4), ompC (uniprotkb:P06996), ompA (uniprotkb:P0A910), atpA (uniprotkb:P0ABB0), atpD (uniprotkb:P0ABB4), adhE (uniprotkb:P0A9Q7),hflB (uniprotkb:P0AAI3), hflC (uniprotkb:P0ABC3), hflK (uniprotkb:P0ABC7), lacI (uniprotkb:P03023), gapA (uniprotkb:P0A9B2), rbsB (uniprotkb:P02925), sdhA (uniprotkb:P0AC41), rho (uniprotkb:P0AG30), udp (uniprotkb:P12758), nuoC (uniprotkb:P33599), treB (uniprotkb:P36672) and manX (uniprotkb:P69797) by cross-linking studies (MI:0030)

MINT-6477988:

yidC (uniprotkb:P25714) physically interacts (MI:0218) with hflB (uniprotkb:P0AAI3), hflK (uniprotkb:P0ABC7), hflC (uniprotkb:P0ABC3), secD (uniprotkb:P0AG90) and secG (uniprotkb:P0AG99) by cross-linking studies (MI:0030)

MINT-6478012:

yidC (uniprotkb:P25714) physically interacts (MI:0218) with hflC(uniprotkb:P0ABC3) and hflK (uniprotkb:P0ABC7) by cross-linking studies (MI:0030)

Abbreviations: IMP, inner membrane protein, TM, transmembrane segment

Keywords: FtsH, HflK, HflC, SecYEG, YidC