The Lhcb protein and xanthophyll composition of the light harvesting antenna controls the ΔpH-dependency of non-photochemical quenching in Arabidopsis thaliana

Abstract 

Nonphotochemical quenching (NPQ) is the photoprotective dissipation of energy in photosynthetic membranes. The hypothesis that the ΔpH-dependent component of NPQ (qE) component of non-photochemical quenching is controlled allosterically by the xanthophyll cycle has been tested using Arabidopsis mutants with different xanthophyll content and composition of Lhcb proteins. The titration curves of qE against ΔpH were different in chloroplasts containing zeaxanthin or violaxanthin, proving their roles as allosteric activator and inhibitor, respectively. The curves differed in mutants deficient in lutein and specific Lhcb proteins. The results show that qE is determined by xanthophyll occupancy and the structural interactions within the antenna that govern allostericity.

Abbrevations: 9aa, 9-aminoacridine, DES, de-epoxidation state of the xanthophyll cycle pool, LHCII, light harvesting complexes of photosystem II, Lhcb, the proteins of LHCII, NPQ, non-photochemical quenching, ΔpH, pH difference across the thylakoid membrane, PSII, photosystem II, q9aa, quenching of 9aa fluorescence, qE, the ΔpH-dependent component of NPQ, WT, wild-type, zea, zeaxanthin

Keywords: Xanthophyll cycle, Thylakoid membrane, Non-photochemical quenching, Light harvesting complex, Lutein, Arabidopsis