FEBS Letters
Volume 582, Issue 12 , Pages 1661-1666, 28 May 2008

A comparative metabolomic study of NHR-49 in Caenorhabditis elegans and PPAR-α in the mouse

Edited by Laszlo Nagy

  • Helen J. Atherton

      Affiliations

    • The Department of Biochemistry, Tennis Court Road, University of Cambridge, Cambridge CB2 1QW, UK
    • ,
  • Oliver A.H. Jones

      Affiliations

    • The Department of Biochemistry, Tennis Court Road, University of Cambridge, Cambridge CB2 1QW, UK
    • ,
  • Shahid Malik

      Affiliations

    • Chenomx, Suite 800, 10050 – 112 Street, Edmonton, Alberta T5K 2J1, Canada
    • ,
  • Eric A. Miska

      Affiliations

    • Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    • ,
  • Julian L. Griffin

      Affiliations

    • The Department of Biochemistry, Tennis Court Road, University of Cambridge, Cambridge CB2 1QW, UK
    • Corresponding Author InformationCorresponding author. Fax: +44 0 1223 333 345.

Received 19 March 2008; accepted 13 April 2008. published online 22 April 2008.

Abstract 

Proton Nuclear Magnetic Resonance spectroscopy and Gas Chromatography Mass Spectrometry based metabolomics has been used in conjunction with multivariate statistics to examine the metabolic changes in Caenorhabditis elegans following the deletion of nuclear hormone receptor-49 (nhr-49). Deletion of the receptor produced profound changes in fatty acid metabolism, in particular an increase in the ratio of unsaturated to saturated fatty acids, a decrease in the concentration of glucose and increases in lactate and alanine. Given the proposed functional similarity between nhr-49 and the mammalian peroxisome proliferator-activated receptors (PPARs) these changes were compared with the metabolome of the PPAR-α null mouse. The metabolomic approach demonstrated a number of similarities including the regulation of lipid synthesis, β-oxidation of fatty acids and changes in glycolysis/gluconeogenesis.

Keywords: Peroxisome proliferator-activated receptors, Functional genomics, Nuclear hormone receptors, NMR spectroscopy, Gas chromatography mass spectrometry

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PII: S0014-5793(08)00338-4

doi:10.1016/j.febslet.2008.04.020

FEBS Letters
Volume 582, Issue 12 , Pages 1661-1666, 28 May 2008

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