Investigating the nucleic acid interactions and antimicrobial mechanism of buforin II

Abstract 

Buforin II (BF2) is an antimicrobial peptide that is hypothesized to kill bacteria by entering cells and binding nucleic acids. To further investigate this proposed mechanism, we used computer modeling and experimental measurements to consider the interactions between BF2 and DNA. Computational and experimental results imply that the peptide forms specific interactions with DNA. Moreover, we observe a general correlation between DNA affinity and antimicrobial activity for a series of BF2 variants. Thus, our results support the proposed mechanism for BF2 and provide a useful approach for evaluating the nucleic acid interactions of other antimicrobial peptides.

Abbreviations: BF2, buforin II, MD, molecular dynamics, FID, fluorescent intercalator displacement, MM-GBSA, molecular mechanics-Generalized Born surface area

Keywords: Histone-derived antimicrobial peptide, Buforin II, Parasin, Hipposin, Peptide–DNA interaction, Molecular dynamics