Interactions between PBEF and oxidative stress proteins – A potential new mechanism underlying PBEF in the pathogenesis of acute lung injury

Abstract 

Identification of pre-B-cell colony-enhancing factor (PBEF) interacting partners may reveal new molecular mechanisms of PBEF in the pathogenesis of acute lung injury (ALI). The interactions between PBEF and NADH dehydrogenase subunit 1(ND1), ferritin light chain and interferon induced transmembrane 3 (IFITM3) in human pulmonary vascular endothelial cells were identified and validated. ND1, ferritin and IFITM3 are involved in oxidative stress and inflammation. Overexpression of PBEF increased its interactions and intracellular oxidative stress, which can be attenuated by rotenone. The interaction modeling between PBEF and ND1 is consistent with the corresponding experimental finding. These interactions may underlie a novel role of PBEF in the pathogenesis of ALI.

Structured summary

Abbreviation: ALI, acute lung injury, ARDS, acute respiratory distress syndrome, A2aR, adenosine A2a receptor, ETC, electron transport chain, EC, endothelial cells, HPAEC, human primary pulmonary artery endothelial cells, HLMVEC, human primary lung microvascular endothelial cells, IFITM3, interferon induced transmembrane protein 3, ND1, NADH dehydrogenase subunit 1, PBEF, Pre-B-cell colony-enhancing factor, UCE2L6, γ-glutamyl transferase and ubiquitin conjugating enzyme E2L6, ROS, reactive oxygen species

Keywords: PBEF, Interaction, Vascular permeability, Acute lung injury, Inflammation, Oxidative stress