AML1-ETO interacts with Sp1 and antagonizes Sp1 transactivity through RUNT domain

Abstract 

AML1-ETO fusion protein is observed in approximately 12% of acute myeloid leukemia. In the present research, we found that AML1-ETO is able to inhibit Sp1 transactivity. We also found that this inhibition of Sp1 transactivity by AML1-ETO is achieved by interaction between Sp1 and RUNT domain of AML1. AML1b is able to abrogate the inhibition of AML1-ETO. Since Sp1 is involved in hematopoietic cell differentiation, we proposed that AML1-ETO promotes leukemogenesis by blocking cell differentiation through inhibition of Sp1 transactivity.

Structured summary

Abbreviations: PB, phenylbutyrate, HCK, human hematopoietic cell kinase, AML, Acute myeloid leukemia

Keywords: AML1-ETO, Sp1, Cell differentiation, Acute myeloid leukemia, Transcription regulation