FEBS Letters
Volume 582, Issue 15 , Pages 2173-2177, 25 June 2008

The p53 target protein Wig-1 binds hnRNP A2/B1 and RNA Helicase A via RNA

Edited by Gianni Cesareni

  • Magdalena Prahl

      Affiliations

    • Department of Oncology-Pathology, Cancer Center Karolinska (CCK), Karolinska Institutet, SE-171 76 Stockholm, Sweden
    • ,
  • Anna Vilborg

      Affiliations

    • Department of Oncology-Pathology, Cancer Center Karolinska (CCK), Karolinska Institutet, SE-171 76 Stockholm, Sweden
    • ,
  • Carina Palmberg

      Affiliations

    • Department of Medical Biochemistry and Biophysics (MBB), Karolinska Institutet, SE-171 77 Stockholm, Sweden
    • ,
  • Hans Jörnvall

      Affiliations

    • Department of Medical Biochemistry and Biophysics (MBB), Karolinska Institutet, SE-171 77 Stockholm, Sweden
    • ,
  • Charlotte Asker

      Affiliations

    • Department of Medicine, Clinical Pharmacology Unit 3, Karolinska Institutet, SE-171 76 Stockholm, Sweden
    • ,
  • Klas G. Wiman

      Affiliations

    • Department of Oncology-Pathology, Cancer Center Karolinska (CCK), Karolinska Institutet, SE-171 76 Stockholm, Sweden
    • Corresponding Author InformationCorresponding author. Fax: +46 8 32 10 47.

Received 25 October 2007; received in revised form 7 April 2008; accepted 25 April 2008. published online 03 June 2008.

Abstract 

The p53-induced Wig-1 gene encodes a double stranded RNA-binding zinc finger protein. We generated Saos-2 osteosarcoma cells expressing tetracycline-inducible Flag-tagged human Wig-1. Induction of Wig-1 expression by doxycycline inhibited cell growth in a long-term assay but did not cause any changes in cell cycle distribution nor increased fraction of apoptotic cells. Using co-immunoprecipitation and mass spectrometry, we identified two Wig-1-binding proteins, hnRNP A2/B1 and RNA Helicase A, both of which are involved in RNA processing. The binding was dependent on the presence of RNA. Our results establish a link between the p53 tumor suppressor and RNA processing via hnRNPA2/B1 and RNA Helicase A.

Structured summary


MINT-6542926, MINT-6542899:

WIG1 (uniprotkb:Q9HA38) physically interacts (MI:0218) with hnRNP A2/B1 (uniprotkb:P22626) by anti bait coimmunoprecipitation (MI:0006)

MINT-6542945:

RHA (uniprotkb:Q08211) physically interacts (MI:0218) with hnRNP A2/B1 (uniprotkb:P22626) by anti bait coimmunoprecipitation (MI:0006)

MINT-6542918, MINT-6542891:

WIG1 (uniprotkb:Q9HA38) physically interacts (MI:0218) with RHA (uniprotkb:Q08211) by anti bait coimmunoprecipitation (MI:0006)

MINT-6542867:

WIG1 (uniprotkb:Q9HA38) physically interacts (MI:0218) with RHA (uniprotkb:Q08211) by anti tag coimmunoprecipitation (MI:0007)

MINT-6542879:

WIG1 (uniprotkb:Q9HA38) physically interacts (MI:0218) with hnRNP A2/B1(uniprotkb:P22626) by anti tag coimmunoprecipitation (MI:0007)

Keywords: Wig-1, p53, dsRNA, RNA Helicase A, hnRNP A2/B1

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PII: S0014-5793(08)00461-4

doi:10.1016/j.febslet.2008.04.065

FEBS Letters
Volume 582, Issue 15 , Pages 2173-2177, 25 June 2008

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