Molecular cloning of novel Monad binding protein containing tetratricopeptide repeat domains

Itsuki, Yuki; Saeki, Makio; Nakahara, Hirokazu; Egusa, Hiroshi; Irie, Yasuyuki; Terao, Yutaka; Kawabata, Shigetada; Yatani, Hirofumi; Kamisaki, Yoshinori

doi:10.1016/j.febslet.2008.05.041

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Volume 582, Issue 16 , Pages 2365-2370, 9 July 2008

Molecular cloning of novel Monad binding protein containing tetratricopeptide repeat domains

Edited by Barry Halliwell

Yuki Itsuki
- Department of Pharmacology, Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
- Department of Fixed Prosthodontics, Graduate school of Dentistry, Osaka University, Japan
Makio Saeki
- Department of Pharmacology, Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
- Corresponding author. Fax: +81 6 6879 2914.
Hirokazu Nakahara
- The First Department of Oral and Maxillofacial Surgery, Osaka University, Japan
Hiroshi Egusa
- Department of Fixed Prosthodontics, Graduate school of Dentistry, Osaka University, Japan
Yasuyuki Irie
- Department of Pharmacology, Iwate Medical University, Morioka, Iwate, Japan
Yutaka Terao
- Department of Oral and Molecular Microbiology, Graduate School of Dentistry, Osaka University, Japan
Shigetada Kawabata
- Department of Oral and Molecular Microbiology, Graduate School of Dentistry, Osaka University, Japan
Hirofumi Yatani
- Department of Fixed Prosthodontics, Graduate school of Dentistry, Osaka University, Japan
Yoshinori Kamisaki
- Department of Pharmacology, Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
- E-Institute of Shanghai Universities, Division of Nitric Oxide and Inflammatory Medicine, Shanghai, China

Received 2 May 2008; received in revised form 28 May 2008; accepted 28 May 2008. published online 05 June 2008.

Abstract

We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-α(TNF-α) and cycloheximide (CHX). By affinity purification and mass spectrometry, we identified RNA polymerase II-associated protein 3 (RPAP3) as a binding protein of Monad. Overexpression of RPAP3 in HEK 293 potentiated caspase-3 activation and apoptosis induced by TNF-α and CHX. In addition, knockdown of RPAP3 by RNA interference resulted in a significant reduction of apoptosis induced by TNF-α and CHX in HEK293 and HeLa cells. These results raise the possibility that RPAP3, together with Monad, may function as a novel modulator of apoptosis pathway.