FEBS Letters
Volume 582, Issue 17 , Pages 2527-2531, 23 July 2008

Zinc regulation of aminopeptidase B involved in neuropeptide production

Edited by Miguel De la Rosa

  • Shin-Rong Hwang

      Affiliations

    • Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Drive, MC 0744, La Jolla, CA 92093-0744, United States
    • ,
  • Vivian Hook

      Affiliations

    • Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Drive, MC 0744, La Jolla, CA 92093-0744, United States
    • Department of Neuroscience, Pharmacology, and Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, United States
    • Corresponding Author InformationCorresponding author. Address: Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Drive, MC 0744, La Jolla, CA 92093-0744, United States. Fax: +1 858 822 6681.

Received 6 May 2008; received in revised form 5 June 2008; accepted 12 June 2008. published online 19 June 2008.

Abstract 

Aminopeptidase B (AP-B) is a metallopeptidase that removes basic residues from the N-termini of neuropeptide substrates in secretory vesicles. This study assessed zinc regulation of AP-B activity, since secretory vesicles contain endogenous zinc. AP-B was inhibited by zinc at concentrations typically present in secretory vesicles. Zinc effects were dependent on concentration, incubation time, and the molar ratio of zinc to enzyme. AP-B activity was recovered upon removal of zinc. AP-B with zinc became susceptible to degradation by trypsin, suggesting that zinc alters enzyme conformation. Zinc regulation demonstrates the metallopeptidase property of AP-B.

Abbreviations: AP-B, aminopeptidase B, ME, (Met)enkephalin

Keywords: Arg/Lys aminopeptidase, Zinc regulation, Inhibition, Neuropeptide, Secretory vesicle

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PII: S0014-5793(08)00507-3

doi:10.1016/j.febslet.2008.06.017

FEBS Letters
Volume 582, Issue 17 , Pages 2527-2531, 23 July 2008

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