Identification of a rare subset of adipose tissue-resident progenitor cells, which express CD133 and TRPC3 as a VEGF-regulated Ca2+ entry channel

Poteser, Michael; Graziani, Annarita; Eder, Petra; Yates, Ameli; Mächler, Heinrich; Romanin, Christoph; Groschner, Klaus

doi:10.1016/j.febslet.2008.06.049

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Volume 582, Issue 18 , Pages 2696-2702, 6 August 2008

Identification of a rare subset of adipose tissue-resident progenitor cells, which express CD133 and TRPC3 as a VEGF-regulated Ca²⁺ entry channel

Edited by Maurice Montal

Michael Poteser
- Institute of Pharmaceutical Sciences, Pharmacology and Toxicology, Karl-Franzens-University of Graz, Graz, Austria
Annarita Graziani
- Institute of Pharmaceutical Sciences, Pharmacology and Toxicology, Karl-Franzens-University of Graz, Graz, Austria
Petra Eder
- Institute of Pharmaceutical Sciences, Pharmacology and Toxicology, Karl-Franzens-University of Graz, Graz, Austria
Ameli Yates
- Department of Cardiac Surgery, Medical University Graz, Graz, Austria
Heinrich Mächler
- Department of Cardiac Surgery, Medical University Graz, Graz, Austria
Christoph Romanin
- Department of Biophysics, University of Linz, A-4020 Linz, Austria
Klaus Groschner
- Institute of Pharmaceutical Sciences, Pharmacology and Toxicology, Karl-Franzens-University of Graz, Graz, Austria
- Corresponding author. Fax: +43 316 380 9890.

Received 2 May 2008; received in revised form 16 June 2008; accepted 26 June 2008. published online 04 July 2008.

Abstract

VEGF-induced Ca²⁺ signalling was investigated in CD133⁺/VEGFR-2⁺ progenitor cells isolated from human adipose stroma. Colonies derived from CD133⁺ immunoselected cells displayed inhomogenous Ca²⁺ signals, with variable magnitude of VEGF-induced Ca²⁺ entry, which positively correlated with expression of the Ca²⁺ channel protein TRPC3. High levels of VEGF-induced Ca²⁺ entry and TRPC3 expression were preferentially detected in rim areas of expanding colonies. Dominant negative suppression of TRPC3 inhibited VEGF-induced Ca²⁺entry into CD133⁺ cells. Our results identify TRPC3 as a key Ca²⁺ entry channel in a subset of CD133⁺ stem cells. We suggest TRPC3 as an essential determinant of cell fate in CD133⁺ progenitor-derived colonies.