Dehydroascorbate and glucose are taken up into Arabidopsis thaliana cell cultures by two distinct mechanisms

Horemans, N.; Szarka, A.; De Bock, M.; Raeymaekers, T.; Potters, G.; Levine, M.; Banhégyi, G.; Guisez, Y.

doi:10.1016/j.febslet.2008.07.001

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Volume 582, Issue 18 , Pages 2714-2718, 6 August 2008

Dehydroascorbate and glucose are taken up into Arabidopsis thaliana cell cultures by two distinct mechanisms

Edited by Ulf-Ingo Flügge

N. Horemans
- University of Antwerp, Department of Biology, Plant Physiology, Groenenborgerlaan 171, B-2020 Antwerp, Belgium
- Corresponding author. Fax: +32 3 259 34 17.
A. Szarka
- Department of Applied Biotechnology and Food Science, Laboratory of Biochemistry and Molecular Biology, Belgium
M. De Bock
- University of Antwerp, Department of Biology, Plant Physiology, Groenenborgerlaan 171, B-2020 Antwerp, Belgium
T. Raeymaekers
- University of Antwerp, Department of Biology, Plant Physiology, Groenenborgerlaan 171, B-2020 Antwerp, Belgium
G. Potters
- University of Antwerp, Department of Bioscience Engineering, Groenenborgerlaan 171, B-2020 Antwerp, Belgium
M. Levine
- Molecular and Clinical Nutrition Section, Digestive Diseases Branch, NIDDK, National Institutes of Health, Bethesda, MD 20892-1372, United States
G. Banhégyi
- Pathobiochemistry Research Group of Hungarian Academy of Sciences and Semmelweis University, Hungary
Y. Guisez
- University of Antwerp, Department of Biology, Plant Physiology, Groenenborgerlaan 171, B-2020 Antwerp, Belgium

Received 9 June 2008; received in revised form 2 July 2008; accepted 2 July 2008. published online 09 July 2008.

Abstract

The possible involvement of glucose (Glc) carriers in the uptake of vitamin C in plant cells is still a matter of debate. For the first time, it was shown here that plant cells exclusively take up the oxidised dehydroascorbate (DHA) form. DHA uptake is not affected by 6-bromo-6-deoxy-ascorbate, an ascorbate (ASC) analogue, specifically demonstrating ASC uptake in animal cells. There is no competition between Glc and DHA uptake. Moreover, DHA and Glc carriers respond in the opposite manner to different inhibitors (cytochalasin B, phloretin and genistein). In conclusion, the plant plasma membrane DHA carrier is distinct from the plant Glc transporters.

Abbreviations: ASC, ascorbate, BrASC, 6-bromo-6-deoxy-ASC, DHA, dehydroascorbate, DPI, diphenyleniodonium, Glc, glucose, GLUT, glucose transporter