FEBS Letters
Volume 582, Issue 18 , Pages 2719-2724, 6 August 2008

Ammonium-dependent hydrogen peroxide production by mitochondria

Edited by Peter Brzezinski

  • Vera G. Grivennikova

      Affiliations

    • Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russian Federation
    • ,
  • Gary Cecchini

      Affiliations

    • Molecular Biology Division, VA Medical Center, 4150 Clement Street, San Francisco, CA 94121, USA
    • Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA
    • ,
  • Andrei D. Vinogradov

      Affiliations

    • Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russian Federation
    • Mitoengineering Center of Moscow State University, Moscow 119992, Russian Federation
    • Corresponding Author InformationCorresponding author. Address: Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russian Federation. Fax: +7 495 939 1376.

Received 6 May 2008; received in revised form 27 June 2008; accepted 30 June 2008. published online 10 July 2008.

Abstract 

NADH-supported generation of H2O2 by permeabilized rat heart mitochondria was partially prevented by the specific complex I-directed inhibitor, NADH-OH, and was significantly stimulated by ammonium. Ammonium did not affect H2O2 production by complex I in coupled submitochondrial particles. The soluble mitochondrial matrix protein fraction catalyzed NADH-dependent H2O2 production, which was greatly (∼10-fold) stimulated by ammonium. We conclude that complex I is not the major contributor to mitochondrial superoxide (hydrogen peroxide) generation and that there are specific ammonium-sensitive NADH:oxygen oxidoreductase(s) in the mitochondrial matrix which are responsible for mitochondrial H2O2 production.

Abbreviations: SMP, submitochondrial particles, ClCCP, carbonyl cyanide m-chlorophenyl-hydrazone, FMN, flavin mononucleotide, FAD, flavin adenine dinucleotide

Keywords: Hydrogen peroxide, Mitochondria, Complex I, Ammonium

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PII: S0014-5793(08)00578-4

doi:10.1016/j.febslet.2008.06.054

FEBS Letters
Volume 582, Issue 18 , Pages 2719-2724, 6 August 2008

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